Performance evaluation of the q exactive hfx for shotgun. Q exactive hf x orbitrap lcmsms system higher quailty data, faster than ever. In the q exactive plus tune window, on the toolbar, click the onoffstandby button to take the mass. The structure of q exactive hybrid quadrupoleorbitrap mass spectrometer as shown in figure 2, the q exactive hybrid quadrupoleorbitrap mass spectrometer mainly includes an ion source, a stackedring ion guide slens, a quadrupole mass filter, a curved linear trap ctrap, a higher energy collisional dissociation hcd cell, and an orbitrap. How much orbitrap q exactive plus costs compared to. Agilent technologies 6520 qtof mass spectrometer center. Aias 10 user manuals aias 3000 user manuals triplus rsh user manuals triplus 100 ls user manuals triplus 300 headspace user manuals triplus classic user manuals trace 0 series gc user manuals isq series manuals tsq series manuals legacy ms manuals. The exactive ms detector applies high voltage to the esi probe.
Isobaric mass tagging quantification using q exactive. Agilent automated sample prep for preomics ist kits. The manual describes the installation and operation of the gcappiinterface for the. This q exactive hf software manual provides reference information about the parameters in the instrument configuration window, the instrument setup window, and the q exactive hf tune window. Warning symbols alert the user to check for hazardous conditions. A new peakpicking algorithm, a brighter ion source, and optimized ion transfers enable productive msms acquisition above 40 hz at. Upgrade your maps peptide mapping thermo scientific. Thermo fisher scientific exactive operating manual vii identifying safety information the exactive operating manual contains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed. The proposed q exactive hf orbitrap system will be used for measuring complex mixtures of proteins, peptides, lipids, metabolites and other biomolecules requiring ultrahigh resolution tandem mass spectrometry for detection, identification, and quantitation of those complex mixtures. Thermo scientific q exactive hfx hybrid quadrupoleorbitrap. It is recommended to always load the log directory before 3. The system was operated either in the dda mode or in the dia mode. Which q exactive orbitrap mass spectrometer is best for my lab. A more detailed and thorough account of these procedures can be found in the q exactive operating manual.
About this guide this exactive operating manual describes the modes of operation and principle hardware components of your exactive instrument. Dataindependent acquisition for the orbitrap q exactive hf. Q exactive hybrid quadrupoleorbitrap mass spectrometer support explore our getting started and troubleshooting sections for solutions to top inquiries and common problems. A more detailed and thorough account of these procedures can be found in the qexactive operating manual. Thermo scientific content manual and documents official. Exactive series operating manual revision a thermo fisher. If you have not already done so, choose start programs thermo exactive series tune to start the q exactive plus tune program. Thermo scientific q exactive hfx hybrid quadrupole. How much orbitrap q exactive plus costs compared to orbitrap. Thermo q exactive from 2012 with proxeon thermo easynlc. Consider an excellent book, we remind regarding q exactive plus user manual this is not a new most current book, however this publication is always bearing in mind constantly. For resolving power 17,500, detection time is about 64 ms. Since the q exactive hf, operating with an ms2 resolution setting of 60000 performs the same as the q exactive plus ms, operating at 35000 and to demonstrate using 2 isolation widths 0. Download pdf q exactive plus user manual prodeddloy.
This instrument was installed in 2007 and is equipped with a chipbased chromatographic interface. Sep 16, 2019 it was followed in 2014 with the q exactive hf ms thermo fisher scientific, bremen, germany, which contains the high. Thermo scientific xcalibur qualitative analysis user guide ix p preface this user guide for qualitative analysis describes how to use the thermo xcalibur mass spectrometry data system to identify unknown compounds or carry out a trace analysis. The spectrum window of exactive tune shows a realtime display of the mass spectrum. Generally, there are only li protein groups for both isolation widths. This guide is intended for all personnel who operate the q exactive mass spectrometer. Browse through our guides and tools section to access a comprehensive portfolio of productrelated support resources. The q exactive hf, a benchtop mass spectrometer with a prefilter. Together, these components enable fast identification and analysis of peptides, labelfree. Before reading this manual, read the getting st arted manual for the xcalibur data system and.
The q exactive hf system combines a stateoftheart segmented quadrupole for highperformance precursor ion selection with a highresolution, accuratemass hram ultrahighfield orbitrap mass analyzer to deliver a superior combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. Sciencebased website focusing on orbitrap mass spectrometers with workflows for proteomics, metabolomics, lipidomics, pharmaceuticals, biopharmaceuticals, clinical, drugs of abuse, sports doping, environmental, and food safety for life science and analytical researchers. For information about the operating proc edures for the q exactive hf mass spectrometer, we recommend that you read the exactive series operating. The features of q exactive hybrid quadrupoleorbitrap mass spectrometer. The resolution up to 140,000 to see more peaks in samples and it can improve results reliability when analyzing samples in complex matrices. The q exactive hf x mass spectrometer boosts productivity in protein identification, while maintaining the superb accuracy of the high field orbitrap mass analyzer. High resolution mass spectrometry of antibody drug. Q exactive hf x hybrid quadrupole orbitrap mass spectrometer support q exactive hf. Q exactive hf tune use q exactive hf tune to operate the q exactive hf mass spectrometer via the data system computer.
Choose an option from the help menu or press the f1 key. Product specifications thermo scientific q exactive. Cd insert operating manual online help software manual quick start guide. Exactive quickstart guide thermo fisher scientific. This text is a guide to operating the qexactive mass spectroscopy. This manual should be kept near the instrument to be available for quick reference. The q exactive hf x also enables rapid phosphoproteomics with routine analysis of more than 5000 phosphopeptides with short singleshot 15 min lcmsms measurements, or 16 700 phosphopeptides quantified across ten conditions in six gradient hours using tmt10plex and offline peptide fractionation. Fast scantoscan polarity switching in both ms and msms can find more compounds in. The instruments include 10 of the latest generation of q exactive hf x mass spectrometers exhibiting improved speed and sensitivity, and one orbitrap fusion lumos mass spectrometer providing complementary fragmentation techniques and scanning. Q exactive hfx and waters acquity lc standard operating. Thus, it is mandatory to read also the q exactive operating manual and the ion max and ion maxs api source hardware manual. You can paste this into wordpad and email the spectra to yourself 3. Log in your mass spec qexactive session on the cores.
Thermofisher scientific q exactive hf mass spectrometer. Q exactive mass spectrometer we know you, or someone you know, worked very hard to get your orbitrap based mass spectrometer in your lab and even harder preparing your precious samples. Exactive operating manual thermo fisher scientific. For information regarding the installation, features, functionality, and use of this product, please refer to the following sources of information. The instruments include 10 of the latest generation of q exactive hf x mass spectrometers exhibiting improved speed and sensitivity, and one orbitrap fusion lumos mass spectrometer providing complementary fragmentation techniques and. Thermo scientific q exactive thermo scientific q exactive plus thermo scientific q exactive hf. Operating environment must be 1526 c 5978 f and relative humidity must be 4070% with no condensation weight q exactive plus mass spectrometer. In addition, this manual provi des stepbystep instruct ions for cleaning and maintaining your. To fully take advantage of the parallel fill and detect capabilities of the qexactive, we need to balance the max fill times with the transient length. Log in your ass spec qexactive session on the cores. If necessary, open the tune method file that stores th e factory default tune settings for lowflow esi operation. Thermo scientific q exactive plus orbitrap lcmsms system.
To set up the mass spectrometer in the q exactive tune software for tuning and calibration in the hesims mode 1. Thermo scientific q exactive hf orbitrap lcmsms system. It is recommended to always load the log directory before. The thermo scientific tm q exactive tm plus takes quanfirmation to a. Progress in proteomics is mainly driven by advances in mass spectrometric ms technologies. Operating mode position, the gas pressure is within the operational limit, and the vacuum levels are sufficient for operating the instrument. About this manual this exactive plus operating manual contains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed.
Q exactive hfx hybrid quadrupole orbitrap mass spectrometer. Additionally, the q exactive hf has a compact orbitrap analyzer. Thermo scientific compound discoverer user guide vii system requirements the compound discoverer 2. Fundamentals and advances of orbitrap mass spectrometry. The results for the neat li sample are shown in figure 6. Exactive plus operating manual pn 23060, revision a i. Dataindependent acquisition for the orbitrap q exactive. We are working just as hard to make sure that you have the latest information and all the additional resources you need to produce the highest quality data to. Here we benchmarked the performance of the latest ms instrument in the benchtop orbitrap series, the q exactive hf x, against its predecessor for proteomics applications. The partially automated agilent bravo requires offboard centrifugation.
Jul 11, 2017 the unmatched mass accuracy and resolution achieved by the orbitrap mass analyzer allows full sequence coverage to be achieved confidently, each and every time. The figures above show data generated on the agilent bravo in a partiallyautomated protocol, using 1 l plasma with analysis on a thermo q exactive hf orbitrap. Q exactive hf quickstart guide thermo fisher scientific. The menu bar provides dropdown menus with commands for operating the program. Scientists have long demanded more gcms power for detailed q exactive gc orbitrap gcmsms with autosampler trace gc columns for pesticides. The q exactive hf x also enables rapid phosphoproteomics with routine analysis of more than five thousand phosphopeptides with short singleshot 15minute lcmsms measurements, or 16,700.
The q exactive hf x mass spectrometer uses a highcapacity transfer tube for maximum ion loading, an electrodynamic ion funnel that accommodates and transmits ions over a broader mass range, and a highfield thermo scientific tm orbitrap tm mass analyzer. Many individuals are so pleasant for this, authored by a renowned writer. Ideal for mass spectral analysis for targeted and highthroughput analysis of lowlevel components in complex matrices applications in biopharma, forensic. Q exactive hf software manual thermo fisher scientific. Q exactive hybrid quadrupole orbitrap mass spectrometer. Operating environment must be 1526 c 5978 f and relative humidity must be 4070% with no condensation weight q exactive hf mass spectrometer. Q exactive tune use q exactive tune to operate the q exactive mass spectrometer via the data system computer. Superior ion transmission, together with new scan rates up to 40 hz, drive more efficient use of the orbitrap mass analyzer. I am using a q exactive plus and in the experiment pane for a full ms ddms2 topn there are three settings for the automatic gain control agc, the number of ions to be in the detector orbitrap. The thermo scientific q exactive plus, q exactive hf and q exactive hf x hybrid quadrupoleorbitrap mass spectrometers add superior denatured and native ms intact analysis and subunit topmiddledown analysis capabilities to the most powerful benchtop peptide. Q exactive benchtop quadrupoleorbitrap mass spectrometer search our database for product documents. Acquisition of a q exactive hf hybrid quadrupoleorbitrap. Choose file load tune file to display the open dialog box.
Q exactive mass spectrometer series calibration and. Refer to the exactive series operating manual for instructions about preparing the positive ion mode calibration solution or buying the readytouse solution from. The q exactive hf is ideal for metabolomics youtube. The q exactive plus and q exactive hf mass spectrometers figure 2a have previously been introduced with the protein mode option, which was one of many advancements for intact protein analysis on the orbitrap platform. Q exactive orbitrap mass spectrometers thermo fisher.
Thermo trace gc ultra operating manual the isq gcms series features a new source design ideal for continuous trace 0 gc, trace 10 gc, thermo scientific triplus rsh. The orbitrap fusion series ms, excluding its workbench, weighs over 227 kg 500 lb. Ucsdccms massive datasets mass spectrometry repository. Exactive is a member of the thermo scientific fa mily of advanced mass spectrometer ms detectors. Our hybrid quadrupole orbitrap mass spectrometry systems vary in terms of mass resolution, scan speed and analysis modes. Please note that this guide does not contain any safe ty notices and precautionary statements. Refer to the q exactive gc and exactive gc operating manual for instructions about turning on the gc with vacuum compensation. Scope of this guide the q exactive hf software manual includes the following chapters. Q exactive hybrid quadrupoleorbitrap mass spectrometer.
With its wide dynamic range the q exactive biopharma platform on the q exactive plus and hf instruments provide superior peptide mapping performance within the most complex samples. Up to 50 ms can be used to fill the ctrap with ions and without increasing cycle. Thermo scientific tm q exactive tm hf x hybrid quadrupoleorbitrap tm mass spectrometer rises to the occastion and sets new standards in sensitivity, performans and productivity the q exactive hf x mass spectrometer uses a highcapacity transfer tube for maximum ion loading, an electrodynamic ion funnel that accommodates and transmits ions over a broader mass range, and a highfield thermo. Exactive plus orbitrap mass spectrometer support thermo. Exactive gc tune university of north carolina at chapel hill.
Final excel report is emailed to the user or provided as hardcopy if requested. To view user documentation from the thermo fisher scientific website 1. As a result, the q exactive hf x mass spectrometer takes. Thermo orbitrap fusion series hardware manual pdf download. The q exactive plus and q exactive hf mass spectrometers figure 2a have previously been introduced with the protein mode option, which was one of many advancements for intact protein analysis on the. Q exactive hybrid quadrupoleorbitrap mass spectrometer ms is the most suitable orbitrap platform for this application1,2. This manual should be kept near the instrument to be. Will have to change settings to print landscape orientation b. The thermo scientific tm q exactive tm hybrid quadrupoleorbitrap mass spectrometer introduced the revolutionary concept of quanfirmation tm.
Q exactive biopharma platform thermo fisher scientific. With its unique spectrum, multiplexing capabilities and faster scan speed, the q exactive ms provides enough scan points for precise quantification even for narrow ultrahighperformance liquid chromatography uhplc. It also describes the modes of operation and principle hardware components of your exactive plus instrument. Realize complete biopharma characterization with the thermo scientific q exactive biopharma platform, which offers distinct operational modes optimized for the top three protein characterization workflows. X ms thermo fisher scientific, bremen, germany, provided improved signal over 3. This q exactive hf software manual is intended for all personnel that need to perform measurements with the q exactive hf mass spectrometer, especially the key operator. Mass spectrometric detection was carried out on a q exactive hf orbitrap mass analyzer thermo scientific, san jose, ca, usa. Agilent technologies 6520 qtof mass spectrometer center for. High resolution mass spectrometry of antibody drug conjugates.
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